By Inge Scharrer, Wolfgang Schramm, G. Auerswald, A. Kurth, J. Oldenburg, W. Schramm, B. Zieger
This ebook includes the contribution to the thirty sixth Hemophilia Symposium, Hamburg 2005. the most themes are epidemiolgy, hemophilia remedy, orthopedic therapy in hemophiliacs, hemostaseologic analysis and pediatric hemostaseology. the quantity is rounded off via various unfastened papers and posters on hemophilia, inhibitors in hemophilia and diagnostics.
Read Online or Download 36th Hemophilia Symposium Hamburg 2005: Epidemiology; Hemophilia Therapy - Management of Bleedings and Inhibitors; Orthopedic Treatment in Hemophiliacs; ... Pediatric Hemostaseology; Free Lectures PDF
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Additional resources for 36th Hemophilia Symposium Hamburg 2005: Epidemiology; Hemophilia Therapy - Management of Bleedings and Inhibitors; Orthopedic Treatment in Hemophiliacs; ... Pediatric Hemostaseology; Free Lectures
J Biol Chem 1996; 271: 1935–40. 5. Fay PJ, Koshibu K. The A2 subunit of factor VIIIa modulates the active site of factor IXa. J Biol Chem 1998; 273: 19049–54. 6. Fay PJ, Scandella D. Human inhibitor antibodies specific for the factor VIII A2 domain disrupt the interaction between the subunit and factor IXa. J Biol Chem 1999; 274: 29826–30. 7. Saenko EL, Yakhyaev AV, Mikhailenko I, Strickland DK, Sarafanov AG. Role of the low density lipoprotein-related protein receptor in mediation of factor VIII catabolism.
3 nM) and mapped it to the region R484F509 based on the ability of a monoclonal antibody 413, which epitope encompasses this region, and the synthetic peptide 484-509 to inhibit FVIII binding to LRP . To identify residues within HCh, critical for FVIII/LRP interaction, we have further accomplished a comprehensive site-directed mutagenesis of the A2 region 484509 and residues spatially close to this region suggested by a three dimensional structure (3D) model of A2 . In competition and surface plasmon resonance (SPR) assays, affinities of A2 mutants K466A, R471A, R484A, S488A, R489A, R490A, H497A and K499A for LRP were found decreased by several fold .
30. Bjorkman S, Berntorp E. Pharmacokinetics of coagulation factors: clinical relevance for patients with haemophilia. Clin Pharmacokinet 2001; 40: 815–32. 31. Bovenschen N, van Stempvoort G, Voorberg J, Mertens K, Meijer AB. Cleavage of factor VIII heavy chain by thrombin increases the affinity for low-density lipoprotein receptorrelated protein (LRP). J Thromb Haemost 2003; Suppl July: OC095. 32. Neels JG, Bovenschen N, Zonneveld A-J, Lenting P. Interaction between factor VIII and LDL receptor-related protein.